Objective: To study anti-oxidation effect of drug-containing serum with Pinus yunnanensis on the damaged rat hepatocytes induced by ethanol. Method: Ten SD rats were selected and randomly divided into normal group and P. yunnanensis group (1 g·kg-1)

with 5 rats in each group

twice ig daily for continuous 3 days

and the drug-containing serum was prepared 8 hours after the last ig medication. The neonatal rats were sacrificed and their normal hepatocytes were separated under sterile conditions for culture. The pre-cultured hepatocytes were divided into normal group

model group

negative group

3 drug-containing serum groups of P. yunnanensis (25%

50%

100%).The normal hepatocytes were pre-treated with different concentrations of-drug-containing serum for 1 h. All the other groups except normal group and negative group received ethanol (100 mmol·L-1) for wound inducing. 8 h after injury

MTT method was used to measure cellular survival rate

and the levels of alanine aminotransferase (ALT)

malondialdehyde (MDA)

superoxide dismutase (SOD)

and glutathione (GSH) in culture medium were measured. Result: Compared with the normal group

the hepatocytes in injured rats had significantly higher survival rate

significantly reduced ALT activity and MDA content in culture medium

and significantly increased SOD activity and GSH content (P<0.05

P<0.01) after treatment with 5% and 10% drug-containing serum. Conclusion: The drug-containing serum with P. yunnanensis has anti-oxidation effect on the damaged rat hepatocytes induced by ethanol.