Objective: To observe the intervention effect and mechanism of compatibility of salvianolic acid B

C on renal interstitial fibrosis in rats induced by unilateral ureteral obstruction(UUO). Method: Fiftyhealthy SD male rats were randomly divided into 5 groups:normal group

model group

salvianolic acid B group (12.5 mg·kg-1)

salvianolic acid C group (12.5 mg·kg-1)

salvianolic acid B+C group (6.25 mg·kg-1+6.25 mg·kg-1). Except the normal group

UUO rats models were established. Relevant treated groups were given with relevant drugs for 14 d since day 2 of modeling. Enzyme-linked immunosorbant assay(ELISA) method was used to detect β2-microglobulin(β2-MG) and N-acetyl-β-D-glucosaminidase(NAG). Type I collagen (ColⅠ)

type Ⅲ collagen (Col Ⅲ) and α-smooth muscle actin (α-SMA) by immunohistochemistry staining. Result: ①Renal tubular functional results of rats in each group:compared with normal group

β2-MG and NAG levels were significantly increased in model group and various treatment groups(P<0.05); compared with the model group

the levels of β2-MG and NAG in various treatment groups were significantly decreased (P<0.05). ②Results of immunohistochemistry in nephridial tissue:the levels of ColⅠ

Col Ⅲ

α-SMA in model group were significantly higher than those in the normal group (P<0.05). The levels of ColⅠ

Col Ⅲ in salvianolic acid B

B+C group and α-SMA in treatment groups were significantly lower than those in model group (P<0.05). The levels of ColⅠin salvianolic acid B group

and B+C group were significantly lower than those in salvianolic acid C group (P<0.05). The levels of Col Ⅲ

α-SMA in salvianolic acid B+C group were significantly lower than those in salvianolic acid C group (P<0.05). Conclusion: Compatibility of salvianolicacid B

C may protect renal function

improve tubular function to a certain degree. The mechanism may interfere with UUO rats kidney with the secretion of ColⅠ

Col Ⅲ and α-SMA.