Objective: To preliminarily screen out the pharmacodynamic material basis of Banxia Xiexin Tang in the treatment of gastritis

and establish the content determination method for the pharmacodynamic material basis. Method: Macroporous adsorption resin chromatography (eluting solvent:water

30% ethanol

70% ethanol) was adopted to separate three parts of Banxia Xiexin Tang liquid. Totally 48 healthy Wistar rats (half male and half female) were randomly divided into six groups:the normal group

the model group

the Banxia Xiexin Tang group

the water elution group (the distilled water group for short)

the 30% ethanol elution group (the 30% group for short) and the 70% ethanol elution group (the 70% group for short). Except for the normal group

the experimental animal model of acute gastritis was established with absolute ethyl alcohol with the dosage of 5 mL·kg-1

ig. In addition

60 healthy Wistar rats (half male and half female) were randomly divided into six groups

with 10 in each group

the same as above. Except for the normal group

the remaining five groups were included in the chronic gastritis model by using the comprehensive modeling method

in order to compare the therapeutic effect of the separate parts in treating experimental acute and chronic gastritis. Result: A better therapeutic effect in treatment of gastritis was found in 70% ethanol elution liquid;the gastric mucosal surface was pale pink and smooth

with no obvious ulcer

with a significant difference in ulcer inhibition rate in comparison with the model group (P<0.05). The content of baicalin and berberine hydrochloride were determined by HPLC method in 70% ethanol elution parts. Conclusion: The efficacy of active substance of Banxia Xiexin Tang mostly existed in the 70% ethanol elution parts separated by macroporous adsorption resin

its active component for treating gastritis are related to alkaloids

flavonoids.