Objective: To investigate content change of main ingredients in Periplaneta americana by mechanical pressing

and investigate protective effect of pressed P. americana on liver injury in mice induced by CCl4. Method: Oil in P. americana was removed by mechanical pressing

ninhydrin colorimetry and phenol-sulfuric acid method were used to measure contents of total amino acids and total polysaccharides in P. americana

respectively.Efficacy of pressed P. americana was investigated by acute liver injury model of mice induced with CCl4. Result: Content of oil in pressed P. americana was 8.5%

contents of total amino acids and total polysaccharides were 13.334

21.06 mg·g-1

respectively.Pressed P. americana can significantly reduce liver weight coefficient of mice with liver injury and significantly decrease contents of alanine aminotransferase and aspartate aminotransferase in serum of mice. Conclusion: After processing attenuated

processed product of P. americana has efficacy of anti-liver injury.