Objective: To develop a UPLC-MS/MS method for the determination of adenosine

cytidine

guanosine

mannitol

adenine and cordycepin in Chinese Caterpillar Fungus. Method: Isocratic elution was carried out with methanol-0.1% formic acid as the mobile phase using liquid chromatography-mass spectrometry technology. The separation was performed on Agilent ZORBAX SB-C18

and the mass spectrometer was operated in the positive ionization electrospray (ESI) mode using multiple monitoring (MRM) for analysis of six components. Result: The linear ranges of adenosine

cytidine

guanosine

mannitol

adenine and cordycepin were 37.5-1 200 μg·L-1(r=0.998 5)

20-640 μg·L-1(r=0.996 4)

40-1 280 μg·L-1(r=0.999 1)

77.5-2 480 μg·L-1(r=0.993 5)

27.5-880 μg·L-1(r=0.992 4)

90-2 880 μg·L-1(r=0.993 7) respectively. The recoveries of six analytes ranged from 92.1% to 102.6% and the relative standard deviations were all below 8.1%. The average contents of adenosine

cytidine

guanosine

mannitol

adenine and cordycepin were 268.33

11.28

185.66

1 421.44

10.67

812.21 μg·g-1

respectively. Conclusion: A sensitive

accuracy and suitable UPLC-MS/MS method has been developed

and the method could be applied for the determination of adenosine

cytidine

guanosine

mannitol

adenine and cordycepin in Chinese Caterpillar Fungus.