Objective: To establish the HPLC fingerprint of anti-liver fibrosis active peptides in Trionycis Carapax

reflect the chemical information comprehensively and provide evidence for quality control. Method: HPLC was used on ZORBAX SB-C18(4.6 mm×250 mm

5 μm) with acetonitrile-0.03% trifluoroacetic acid the mobile phase of gradient elution

detection wavelength was set at 220 nm

with the flow rate of 1 mL·min-1

the column temperature was of 25 ℃. Result: HPLC fingerprint of anti-liver fibrosis active peptides in Trionycis Carapax was established

which showed 13 comon peaks. The similarity was evaluated on the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine. The similarities of 10 batches were over 0.9

which indicated that the quality of Trionycis Carapax active peptides was relatively stable. Conclusion: The method is simple and has good precision

reproducibility and stability

effective in quality control of Trionycis Carapax active peptides

and provide quality assurance for further pharmacological studies and preparation research.