Objective: To research Jiedu Quyu decoction effects on human breast cancer cell of invasion ability and mechanism. Method: Respectively

0.625

1.25

2.50 g·L-1 Jiedu Quyu decoction were treated with human breast cancer MCF-7 cell

adhesion assay and transwell assay was performed to test the adhesion ability and invasion ability. RT-PCR and Western blot were used to analyze the expression changes of genes and proteins

including E-cadherin

N-cadherin

CD44

extracellular signal-regulated kinase(ERK)

protein kinase B(Akt). Result: Compared with the blank group

treated with 0.625

1.25

2.50 g·L-1 Jiedu Quyu decoction

the adhesion and invasion ability of MCF-7 can be inhibited obviously. The expression of CD44 and E-cadherin were up-regulated

while the N-cadherin were down-regulated. And the signaling pathway of PI3K/Akt and MEK/ERK were inhibited(P<0.05

P<0.01). Conclusion: Jiedu Quyu decoction could inhibit the invasion of MCF-7.Its mechanism may be correlated with inhibition of heterogeneous adhesion protein expression

and inhibition of epithelial-mesenchymal transition and regulation of signaling pathway.