Objective: Shenfu injection is a compound preparation of traditional Chinese medicine

which is comprised of Ginseng Radix et Rhizoma Rubra and Aconiti Lateralis Radix Praeparata.Its main clinical indications of congestive heart failure

ischemic stroke had been widely accepted bymedical community.Based on ischemic stroke

system biology and experimental research was adopted to explain functional mechanisms of Shenfu injection regulating biological network. Method: Departure of 28 compounds in Shenfu injection

Research used combined methods of TCMGeneDIT database and Agilent literature search (ALS) system addition to Pharmmapper reverse docking to dig the protein information of Shenfu injection to biuld multi-compounds protein network.Protein interaction network was built based on protein-protein interaction extracted from Genecards

BIND

BioGRID

IntAct

Mint database.Extracted significant difference subnetwrok from the netwrok and subjected it to Western blot verification. Result: The whole 28 compounds had connections with 55 proteins

while protein interaction network was built with none-isolated nodes of 53 proteins

from which one subnetwork was extracted with significant difference.Fifteen key proteins in subnetwork were mapped on Biocart signaling pathway

which had relationship with NF-κB signaling pathway

AKT signaling pathway

Toll signaling pathway

MAPK signaling pathway

etc.Western blot confirmed Shenfu injection could attenuate phosphorylation expression of NF-κB p65

IκB-α on ischemia 1 h reperfusion 24 h MCAO model. Conclusion: Computational system biology and experimental study approach preliminary explores molecular mechanism of Shenfu injection for preventing and curing diseases

it can provide references for researches on Chinese medicines.