脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响

谭辉; 王键; 尹婷婷; 胡建鹏; 何玲; 徐伟

doi:10.13422/j.cnki.syfjx.2015220021

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脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响

新安医学 | 更新时间：2024-01-04

- 脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响
- Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats
- 中国实验方剂学杂志 2015年21卷第22期页码：21-25
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2012BAI26B00);国家中医药管理局安徽新安王氏内科流派传承工作室建设项目;安徽中医药大学自然科学研究基金项目(2014zr001)
- DOI：10.13422/j.cnki.syfjx.2015220021
  中图分类号： R285.5
- 纸质出版日期：2015
- 稿件说明：
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谭辉, 王键, 尹婷婷, 等. 脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响[J]. 中国实验方剂学杂志, 2015,21(22):21-25.

TAN Hui, WANG Jian, YIN Ting-ting, et al. Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 21-25.
谭辉, 王键, 尹婷婷, 等. 脑络欣通方对脑缺血大鼠血管再生及局部脑血流量的影响[J]. 中国实验方剂学杂志, 2015,21(22):21-25. DOI： 10.13422/j.cnki.syfjx.2015220021.

TAN Hui, WANG Jian, YIN Ting-ting, et al. Influence of Naoluo Xintong Prescription on Angiogenesis and rCBF in MCAO/R Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 21-25. DOI： 10.13422/j.cnki.syfjx.2015220021.

摘要

目的:探讨脑络欣通方对大脑中动脉闭塞模型大鼠缺血再灌注(MCAO/R)损伤后大鼠血管新生及局部脑血流量(rCBF)的影响。方法:Wistar大鼠90只随机分为假手术组、模型组和脑络欣通组

采用线栓法制作MCAO/R模型

每组分为3

14 d共3个时间点的亚组

酶联免疫吸附试验(ELISA)检测脑组织匀浆中内皮素(ET)和血小板生成素(TPO)水平

免疫组化法检测脑组织中血管内皮生长因子(VEGF)的表达

运用激光多普勒仪动态观察rCBF。结果:与假手术组比较

模型组TPO和ET水平明显升高

VEGF蛋白表达增加

rCBF降低;与模型组比较

脑络欣通组TPO和ET水平显著降低

VEGF蛋白表达增加

rCBF明显恢复;同组内比较

脑络欣通组第7天和第14 天较第3天TPO水平显著下降

第7天和第14天ET水平较第3天明显下降

第14天较第3天VEGF蛋白表达增加

第14天rCBF较第3天显著增加。结论:脑络欣通冻干粉通过调节模型大鼠凝血纤溶调节因子和VEGF

促进血管新生而改善血流状态

提高局部脑血流量来发挥对缺血性脑血管病的治疗作用。

Abstract

Objective: To discuss influence of Naoluo Xintong prescription on angiogenesis and rCBF in MCAO/R rats. Method: Wistar rats were randomly divided into sham group

model group and Naoluo Xintong group

model of MCAO/R rats was reproduced in accordance to suture method

and further were subdivided into three time points

such as 3

14 d.The content of thrombopoietin (TPO) and endothelin (ET) in brain tissue homogenate were determined by ELISA.Expressing of vascular endothelial growth factor (VEGF) protein in cerebrum was assayed by immunohistochemistry.The rCBF was dynamically measured by laser Doppler flowmeter. Result: Compared with sham group

the content of TPO and ET

VEGF protein significantly increased

rCBF reduced in Naoluo Xintong group.Compared with model group

the content of TPO and ET markedly reduced

the amount of VEGF protein and rCBF increased in Naoluo Xintong group.Compared within the same group

the content of TPO in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d

the content of ET in Naoluo Xintong group in 7 d and Naoluo Xintong group in 14 d markedly reduced by comparing with Naoluo Xintong group in 3 d.The amount of VEGF protein in Naoluo Xintong group in 14 d increased by comparing with Naoluo Xintong group in 3 d.The rCBF in Naoluo Xintong group in 14 d markedly increased by comparing with Naoluo Xintong group in 3 d. Conclusion: Naoluo Xintong lyophilized powder can improve blood flow status

increase rCBF by regulating regulatory factor related to cruor

fibrinolysis

VEGF and promoting angiogenesis

which play an treatment role on ischemic cerebral vascular disease (ICVD).

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