Objective: To develop an HPLC method for chlorogenic acid

geniposide

scutellarin

glycyrrhizic acid and schisandrin in Yinshanlian granules. Methods: The analysis was performed on a Wonda SilTM-C18 column(4.6 mm×250 mm

5μm) with gradient elution(0-5 min

86%B;5-35 min

86%-20%B;35-45 min

20%B)by using the mobile phase of acetonitrile(A)-0.4%phosphoric acid solution(B).The flow rate was 1.0 mL·min-1 and the column temperature was maintained at 30℃

the detection wavelengths were set at 325 nm(for chlorogenic acid)

240 nm(for geniposide)

335 nm(for scutellarin)

250 nm(for glycyrrhizic acid) and 220 nm(for schisandrin)

respectively. Result:The linear ranges of chlorogenic acid

geniposide

scutellarin

glycyrrhizic acid and Schisandrinfell within the ranges of 0.0085-0.170μg(r=0.9995)

0.0462-0.924μg(r=0.9990)

0.0216-0.432μg(r=0.9992)

0.0197-0.394μg(r=0.9993)

0.0045-0.090μg(r=0.9999).The average recoveries of were 95.8%-98.4%.The contents of chlorogenic acid

geniposide

scutellarin

glycyrrhizic acid and schisandrin in three batches of samples were 1.105-1.134

5.139-5.204

2.546-2.603

2.208-2.231

0.352-0.361 mg·g-1. Conclusion:This method is consistent with the method validation requirements

and can simultaneously determine the above 5 index components of Yinshanlian granules.