Objective: To establish a new method for quantitative analysis on multi-components by single marker (QAMS) and validate its feasibility and technical adaptability in analysis on Quyu Huatan Tongmai granules for simultaneous determination of 12 main constituents. Method: Quyu Huatan Tongmai granules was chosen as research object

taking berberine hydrochloride as index component

relative correction factors of sodium danshensu

salvianolic acid B

cryptotanshinone

tanshinoneⅠ

tanshinoneⅡA

ferulicacid

epiberberine

coptisine

palmatine hydrochloride

chlorogenic acid and lovastatin were established by HPLC and calculated to achieve QAMS.Contents of 12 components in samples were determined with the external standard method

difference between two methods was compared and to validate correctness and adaptability of QAMS. Result: No significant differences were observed between quantitative results of these two methods with RSD<5%. Conclusion: This established QAMS method can be used for quantitative determination of Quyu Huatan Tongmai granules.It can provide references for quality control of Chinese patent medicine.