Objective: To establish a quantitative assay of multi-components by single marker (QAMS) for determination of four bioactive components in Lancea tibetica

and examine the accuracy and feasibility of the relative correction factors (RCFs) between different types of compounds. Method: L. tibetica was chosen as the research object

and RCFs were determined between acteoside and catalpol

phillyrin and phillygenol. The contents of the four constituents were also calculated according to the RCFs to carry out QAMS. Difference of QAMS and external standard method was investigated to validate the accuracy and feasibility of the QAMS method. Result: RCFs between acteoside and catalpol

phillyrin and phillygenol were 7.0449

2.1350 and 0.9647 respectively within a certain linear range;repeatability was good in different experimental conditions (RSDs were 0.3%

1.2% and 1.2% respectively);there was no significant differences between the quantitative results of QAMS method and external standard method for 4 constituents. Conclusion: It is accurate and feasible to evaluate 4 constituents of L. tibetica by QAMS. Therefore

this method is suitable to determine different types of components

and can provide a new reference for the quality assessment of Tibetan herb-medicine.