氧化苦参碱保护醛固酮诱导的心肌细胞损伤作用机制分析

刘小红; 杨李强; 徐旖旎; 张彦燕; 潘迪; 张蓓; 陈妍; 陶玲; 沈祥春

doi:10.13422/j.cnki.syfjx.2015220108

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氧化苦参碱保护醛固酮诱导的心肌细胞损伤作用机制分析

氧化苦参碱与艳山姜挥发油 | 更新时间：2024-01-04

- 氧化苦参碱保护醛固酮诱导的心肌细胞损伤作用机制分析
- Protective Effects of Oxymatrine on Cardiomyocytes Injury Induced by Aldosteron
- 中国实验方剂学杂志 2015年21卷第22期页码：108-111
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81173586,81560588);教育部新世纪人才支持计划项目(NCET-13-0747);贵州省省长资金项目(黔科教办[2011]28号);贵州省科技基金重点项目(黔科合JZ字[2015]2002);贵州省高等教育科技创新团队项目(黔教合人才团队字[2014]31);贵州省高等学校创新能力提升计划项目(黔教合协同创新字[2013]04);贵州省高层次创新型人才项目(黔科合人才[2015]4029);贵州省科技创新团队项目(黔科合人才团队[2015]4025号)
- DOI：10.13422/j.cnki.syfjx.2015220108
  中图分类号： R285
- 纸质出版日期：2015
- 稿件说明：
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刘小红, 杨李强, 徐旖旎, 等. 氧化苦参碱保护醛固酮诱导的心肌细胞损伤作用机制分析[J]. 中国实验方剂学杂志, 2015,21(22):108-111.

LIU Xiao-hong, YANG Li-qiang, XU Yi-ni, et al. Protective Effects of Oxymatrine on Cardiomyocytes Injury Induced by Aldosteron[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 108-111.
刘小红, 杨李强, 徐旖旎, 等. 氧化苦参碱保护醛固酮诱导的心肌细胞损伤作用机制分析[J]. 中国实验方剂学杂志, 2015,21(22):108-111. DOI： 10.13422/j.cnki.syfjx.2015220108.

LIU Xiao-hong, YANG Li-qiang, XU Yi-ni, et al. Protective Effects of Oxymatrine on Cardiomyocytes Injury Induced by Aldosteron[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(22): 108-111. DOI： 10.13422/j.cnki.syfjx.2015220108.

摘要

目的:研究氧化苦参碱(OMT)抑制细胞外信号调节激酶ERK1/2磷酸化对醛固酮(ALD)诱导心肌细胞损伤的干预作用。方法:采用胰酶消化与差数贴壁分离纯化原代心肌细胞

免疫细胞化学分析心肌细胞纯度。实验分为空白组、模型组(1×10-5 mol·L-1 ALD)和OMT高(3.78×10-4 mol·L-1)

低剂量(1.89×10-4 mol·L-1)组共4组。运用MTT法检测细胞存活率

LDH外漏法测定细胞膜损伤程度

蛋白免疫印迹法分析p-ERK1/2及ERK1/2蛋白表达

实时荧光定量PCR分析ERK mRNA表达。结果:OMT可抑制ALD诱导的细胞存活率降低和LDH外漏增加;与模型组比较

OMT预处理后p-ERK1/2蛋白表达明显下调。结论:OMT对ALD诱导的心肌细胞损伤具有保护作用

其作用与抑制ERK1/2蛋白磷酸化有关。

Abstract

Objective: To observe effect of oxymatrine (OMT) on expression of extracellular signal-regulated kinase 1/2 (ERK1/2) in cardiomyocytes induced by aldosterone (ALD). Method: Pancreatic enzyme digestion method was used to extract caridac myocytes and purity of cultured caridac myocytes was evaluated by immunocytochemistry.Cardiac myocytes were pre-incubated with oxymatrine of high-does and low-does for 1 h

and then exposed to ALD for 24 h.Cell injury was evaluated by MTT assay and LDH leakage.Protein expression of ERK1/2 and phosphorylation-ERK1/2 (p-ERK1/2) was detected by Western blot and ERK mRNA was detected by real time polymerase chain reaction (PCR). Result: After co-incubation with OMT could enhance MTT volume and decrease LDH activity in medium induced by ALD

there was significant difference by comparing with model group.Meanwhile

expression of p-ERK1/2 was significantly down-regulated after co-incubation with OMT. Conclusion: OMT has an protective effect on caridac myocytes induced by ALD

its mechanism may be related to inhibition of p-ERK1/2.

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