Objective: To identify intestinal adsorption ingredients of Epimedii Folium and investigate their absorption characteristics in different intestinal segments. Method: Everted intestinal sac model was utilized.Intestinal sac fluid samples at different time after administration of high

medium and low concentration of Epimedii Folium extract were collected and five flavonoids in fluid samples were determined by HPLC.Accumulative absorbed doses(Q) and absorption rate constants(Ka) of five flavonoids were calculated

while proportions of different flavonoid between Epimedii Folium extract and intestinal absorption liquid were compared. Result: Epimedin A

epimedin B

epimedin C

icariin and icarisid Ⅱ were identified and could be quantitatively analyzed in extract and intestinal sac fluid samples of Epimedii Folium.Q of five flavonoids reached maximum value in front and middle jejunum segments under medium and low concentrations of Epimedii Folium extract

while Q of five flavonoids reached maximum value in ileum under high concentrations of extract.In these three concentrations of Epimedii Folium extract

absorption model of five flavonoids was linearity with R2>0.9

which was in consistent with zero order absorption rate.Ka of five flavonoids increased with increasing of concentration of Epimedii Folium extract

which indicated that intestinal adsorption mechanism of five flavonoids was passive diffusion.Proportions of five flavonoids in intestinal sac fluid were different from those in Epimedii Folium extract. Conclusion: Epimedin A

epimedin B

epimedin C

icariin and icarisid Ⅱ in Epimedii Folium extract can be absorbed in whole intestine.Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Epimedii Folium extract effectively.