HPLC同时测定白癜风丸中羟基红花黄色素A和丹酚酸B的含量

喻录容; 何先元; 黄英如

doi:10.13422/j.cnki.syfjx.2015230037

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HPLC同时测定白癜风丸中羟基红花黄色素A和丹酚酸B的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定白癜风丸中羟基红花黄色素A和丹酚酸B的含量
- Simultaneous Determination of Hydroxysafflor Yellow A and Salvianolic Acid B in Baidianfeng Pills by HPLC
- 中国实验方剂学杂志 2015年21卷第23期页码：37-39
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81373668);重庆市卫生局科技项目(2012-2-144)
- DOI：10.13422/j.cnki.syfjx.2015230037
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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喻录容, 何先元, 黄英如. HPLC同时测定白癜风丸中羟基红花黄色素A和丹酚酸B的含量[J]. 中国实验方剂学杂志, 2015,21(23):37-39.

YU Lu-rong, HE Xian-yuan, HUANG Ying-ru. Simultaneous Determination of Hydroxysafflor Yellow A and Salvianolic Acid B in Baidianfeng Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 37-39.
喻录容, 何先元, 黄英如. HPLC同时测定白癜风丸中羟基红花黄色素A和丹酚酸B的含量[J]. 中国实验方剂学杂志, 2015,21(23):37-39. DOI： 10.13422/j.cnki.syfjx.2015230037.

YU Lu-rong, HE Xian-yuan, HUANG Ying-ru. Simultaneous Determination of Hydroxysafflor Yellow A and Salvianolic Acid B in Baidianfeng Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 37-39. DOI： 10.13422/j.cnki.syfjx.2015230037.

摘要

目的:建立同时测定白癜风丸中羟基红花黄色素A和丹酚酸B含量的测定方法。方法:采用Welchrom C18色谱柱

流动相乙腈-0.1%磷酸梯度洗脱

流速1.0 mL·min-1

检测波长280 nm。结果:羟基红花黄色素A在4.91~36.86 mg·L-1呈良好的线性关系(r=0.9995)

平均回收率99.12%

RSD 0.9%。丹酚酸B在14.99~112.42 mg·L-1呈良好的线性关系(r=0.9997)

平均回收率为98.79%

RSD 1.0%。结论:该方法简便、准确、重复性好

可用于白癜风丸的质量控制。

Abstract

Objective: To establish HPLC method for simultaneous determination of hydroxysafflor yellow A and salvianolic acid B in Baidianfeng pills. Method: The determination was performed on a Welchrom C18 column with acetonitrile-0.1% phosphoric acid as the mobile phase in gradient elution mode at the flow rate of 1.0 mL·min-1. The detection wavelength was 280 nm. Result: Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.91-36.86 mg·L-1 (r=0.9995). The average rate of recovery was 99.12% with RSD 0.9%. Salvianolic acid B presented a good linearity in the concentration range of 14.99-112.42 mg·L-1 (r=0.9997). The average rate of recovery was 98.79% with RSD of 1.0%. Conclusion: This method is simple

accurate and reproducible

and it can be used for quality control of Baidianfeng pills.

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