10家医院饮片组方的茵陈蒿汤中11种成分的含量测定

窦志华; 孟萍; 姜晓燕; 候金燕; 顾薇; 张琳; 王征宇

doi:10.13422/j.cnki.syfjx.2015230044

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10家医院饮片组方的茵陈蒿汤中11种成分的含量测定

化学与分析 | 更新时间：2024-01-04

- 10家医院饮片组方的茵陈蒿汤中11种成分的含量测定
- Determination of 11 Constituents in Yinchenhao Tang Composed by Decoction Pieces from 10 Hospitals of Traditional Chinese Medicine
- 中国实验方剂学杂志 2015年21卷第23期页码：44-49
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省中医药局科技项目(HZ07071)
- DOI：10.13422/j.cnki.syfjx.2015230044
  中图分类号： R284.1
- 纸质出版日期：2015
- 稿件说明：
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窦志华, 孟萍, 姜晓燕, 等. 10家医院饮片组方的茵陈蒿汤中11种成分的含量测定[J]. 中国实验方剂学杂志, 2015,21(23):44-49.

DOU Zhi-hua, MENG Ping, JIANG Xiao-yan, et al. Determination of 11 Constituents in Yinchenhao Tang Composed by Decoction Pieces from 10 Hospitals of Traditional Chinese Medicine[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 44-49.
窦志华, 孟萍, 姜晓燕, 等. 10家医院饮片组方的茵陈蒿汤中11种成分的含量测定[J]. 中国实验方剂学杂志, 2015,21(23):44-49. DOI： 10.13422/j.cnki.syfjx.2015230044.

DOU Zhi-hua, MENG Ping, JIANG Xiao-yan, et al. Determination of 11 Constituents in Yinchenhao Tang Composed by Decoction Pieces from 10 Hospitals of Traditional Chinese Medicine[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(23): 44-49. DOI： 10.13422/j.cnki.syfjx.2015230044.

摘要

目的:建立HPLC同时测定茵陈蒿汤中11种成分含量的方法。方法:采用高效液相色谱法

Symmetry C18色谱柱

流动相乙腈-0.1%磷酸梯度洗脱

流速1.0 mL·min-1

柱温30℃

检测波长254 nm。结果:来源于10家医院的茵陈蒿汤饮片组方中没食子酸、去乙酰车叶草酸甲酯、栀子苷、芦荟大黄素-8-O-葡萄糖苷、大黄酚-1-O-葡萄糖苷、大黄素-8-O-葡萄糖苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的质量分数分别在0.142~1.016

0.518~1.256

9.021~17.214

0.088~0.793

0.097~0.990

0.187~1.587

0.057~0.578

0.147~1.062

0.114~0.730

0.380~1.898和0.375~1.958 mg·g-1。结论:不同来源中药饮片组成的茵陈蒿汤中活性成分的含量存在较大差异。

Abstract

Objective: To establish HPLC method for simultaneously determining 11 constituents in Yinchenhao Tang composed by decoction pieces from 10 hospitals. Method: HPLC was used on Symmetry C18 column (4.6 mm×250 mm

5μm)

with acetonitrile-0.1% phosphoric acid solution as a mobile phase in gradient elution

at a flow rate of 1.0 mL·min-1.The column temperature was 30℃ and detection wave length was set at 254 nm. Result: The mass of gallic acid

deacetylasperulosidic acid methyl ester

geniposide

aloe-emodin-8-O-glucopyranoside

chrysophanol-1-glucoside

emodin-8-glucoside

aloe-emodin

rhein

emodin

chrysophanol and physcion was between 0.142-1.016

0.518-1.256

9.021-17.214

0.088-0.793

0.097-0.990

0.187-1.587

0.057-0.578

0.147-1.062

0.114-0.730

0.380-1.898

0.375-1.958 mg·g-1

respectively. Conclusion: The content of active constituents in Yinchenhao Tang composed by different decoction pieces has significant differences.

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