Objective: To establish the HPLC fingerprint chromatography of Lemai granules and determine the content of six compounds. Method: HPLC-DAD fingerprints of Lemai granules were determined with high-performance liquid chromatography on Phenomenex Luna C18 column (4.6 mm×250 mm

5μm) with the gradient solvent system consisting of methanol (A) and water (B).The flow rate was 1.0 mL·min-1;the detection wavelength was set at 210 nm

and the column temperature was maintained at 30℃. Result: Fingerprints of Lemai granules were obtained. A total of 16 chromatographic peaks were common peaks

and the fingerprints of 10 batches of Lemai granules were identified with high similarity of fingerprints. Six chemical components were identified and their contents were determined. Conclusion: The establishment of HPLC fingerprints provide a scientific basis for specifying medicinal resources and quality evaluation of this formula.