Objective: To study the protective effect of the compounds of taurine

epigallocatechin-3-gallate (EGCG) and genistein on chronic alcohol-induced hepatic fibrosis

and to explore its underlying mechanism. Method: Wistar male rats were randomly divided into six groups:normal group

model group

colchicine group

combination therapy low(taurine 12.5 mg·kg-1+EGCG 1.875 mg·kg-1+genistein 1.25 mg·kg-1)

middle(taurine 25 mg·kg-1+EGCG 3.75 mg·kg-1+genistein 2.5 mg·kg-1)and high (taurine 50 mg·kg-1+EGCG 7.5 mg·kg-1+genistein 5 mg·kg-1) dose group. The rats except normal group were tablished by intragastric administration of alcohol (5.0-9.5 g·kg-1) once a day for 24 weeks and the normal group were given normal saline.Twenty-four hours after the last administration of drugs

all rats were killed

and the hepatic tissue were taken. The contents of hydroxyproline(Hyp)

superoxide dismutase(SOD)

malondialdehyde(MDA)in hepatic tissue and serum biochemical indicators were measured

as well as the related key cytokines/proteins. The damage of liver tissues was observed by light microscope. Result: Compared with normal group

the activities of alanine aminotransferase(ALT)

aspartate aminotransferase(AST)

alkaline phosphatase(ALP)

gamma glutamyl transpeptidase(GGT)

MDA

Hyp

hyaluronic acid(HA)

laminin(LN)

collagen type Ⅲ (PCⅢ)

α-smooth muscle actin(α-SMA)

transforming growth factor-β1(TGF-β1) and mothers against decapentaplegic homolog 3(Smad3) increased (P<0.05) and SOD

glutathione peroxidase (GSH-Px) decreased significantly(P<0.05) in model group;compared with model group

the levels of ALT

AST

ALP

GGT

MDA

Hyp

HA

LN

PCⅢ

α-SMA

TGF-β1 and Smad3 decreased(P<0.05) and SOD

GSH-Px increased significantly(P<0.05) in combination therapy group. Conclusion: The compounds of taurine

EGCG and genistein can relieve chronic hepatic fibrosis induced by alcohol. Its mechanism may involve scavenging free radica

alleviating the lipid peroxidation

inhibiting the expression of α-SMA

TGF-β1 and Smad3.