Objective: To study the effect of Zhenju Jiangya tablet (ZJT) and its Chinese medicine and western medicine components on central nervous system

cardiovascular system

and respiratory system

and then to compare and search for the efficacy of synergism and attenuation. Method: ICR mice were randomly divided into ZJT low-dose

middle-dose and high-dose groups (280

1120

4480 mg·kg-1)

ZJT Chinese medicine part (ZJTZ) low-lose

middle-dose and high-dose groups (274

1095

4380 mg·kg-1)

ZJT western medicine part (ZJTX) low-lose

middle-dose and high-dose groups (6.2

24.9

99.5 mg·kg-1)

normal group

and another diazepam group. Beagle dogs were divided into ZJT low-dose

middle-dose and high-dose groups (60

240

960 mg·kg-1)

ZJTZ low-lose

middle-dose and high-dose groups (58.7

235

939 mg·kg-1)

ZJTX low-lose

middle-dose and high-dose groups (1.3

5.3

21.3 mg·kg-1)

and normal group. ZJT and its traditional Chinese medicine or western medicine part were respectively ig administered to ICR mice or orally administered to Beagle dogs.Spontaneous activity after the treatment in mice

and effect of synergistic subthreshold hypnotic dose of sodium pentobarbital on hypnosis were observed. Changes of systolic pressure

diastolic blood pressure

heart rate

PR interval

QRS complex

QT interval

T wave form

rhythm of heart

breathing rate and breathing depth were recorded and compared before and 0.5

1

2

4

8

24 h after treatment in Beagle dogs. Result: Compared with the normal group

spontaneous activity was reduced in tested mice at doses of 280

1120

4480 mg·kg-1with ig ZJT

with synergistic hypnotic effect with subthreshold hypnotic dose of sodium pentobarbital

shortened sleep latency and prolonged sleep time(P<0.05

P<0.01). The oral administration at dose of 960 mg·kg-1in Beagle dogs reduced systolic pressure

heart rate

breathing rate and breathing depth in tested animals

where the blood pressure began to decrease at 1 h and lasted for 24 h. Prescription studies showed that oral administration of large-dose ZJT could reduce systolic blood pressure

heart rate

breathing rate and breathing depth

with significant sedation effect

mainly due to the western medicine part of the formula. Administration of ZJT could significantly enhance antihypertensive activity

prolong antihypertensive time and prevent PR interval lengthening (P<0.05

P<0.01). Conclusion: The oral administration of high-dose ZJT could decrease systolic blood pressure in tested animals whose extent and dose are positively correlated

and with a certain inhibitory effect on heart rate and respiration. In addition

ZJT has significant sedation effect. The above effect of ZJT mainly come from western medicine part of the formula

and the Chinese medicine part could enhance antihypertensive activity

prolong antihypertensive time and prevent PR interval lengthening

indicating that ZJT characterized by Chinese and western medicine compatibility has certain synergy and attenuation effect.