Objective: To observe the effect of Huatan Tongluo formulae on expression of C/EBP homologous protein(GADD153/CHOP) and C-Jun NH2-terminal Rinases(JNK1) in apoptosis pathway of endoplasmic reticulum stress in rat reconstructive tissue plasminogen activator(rt-PA) after the acute cerebral infarction. Method: The 160 healthy male SD rats were randomly divided into sham group

model group

rt-PA group

rt-PA-Huatan Tongluo group (Chinese medicine group for short). Own embolus method was used to establish rat models of middle cerebral artery embolism (MCAO). The rats in rt-PA group were injected with rt-PA (concentration of 5.67 mg·kg-1)

and those of Chinese medicine group were given with rt-PA and Chinese medicine Huatan Tongluo formulae (7.2 g·kg-1)

2 times a day. RT-PCR was used in four time phases of 6 hours

1 day

3

7 days to detect the expression of GADD153/CHOP and JNK1 mRNA in cerebral cortex infarction tissues in rats. TUNEL method was used to detect brain neurons apoptosis in rats. Result: Compared with sham group

the gene expression of GADD153/CHOP and JNK1 in 4 time phases was significantly higher in model group (P<0.05). Compared with sham group within the same time phase

neurons apoptosis in model group was significantly increased (P<0.05). Compared with the model group

the expression of JNK1 mRNA was decreased in 4 time phases in both rt-PA group and Chinese medicine group (P<0.05)

and rt-PA group could decrease GADD153/CHOP mRNA expression on 1 d while Chinese medicine group could decrease the expression on 6 h and 1 d

with significant difference (P<0.05). Neurons apoptosis in rt-PA group on 1 and 7 d was significantly lower than that in the model group (P<0.05)

while Chinese medicine group decreased neurons apoptosis in various time phases (P<0.05). However

there was no significant difference between rt-PA group and Chinese medicine group. Conclusion: Huatan Tongluo formulae can prevent and treat partially reperfusion injury after acute cerebra accepted thrombolysis by reducing GADD153/CHOP and JNK1 mRNA expression in apoptosis pathway of endoplasmic reticulum stress

and partially inhibiting the apoptosis of nerve cells.