Objective: To observe the intervention effect of ethyl acetate extract of Punica granatum fruit (PGFEAE) on blood glucose and blood lipid in rats with insulin resistance (IR) and normal rats

and evaluate its pharmacological effects on improving insulin resistance. Method: High-sugar and high-fat diet plus intraperitoneal injection of streptozocin(STZ) twice (30 mg·kg-1) to establish rat models of insulin resistance. After 4 weeks of ig administering 100

200

300 mg·kg-1 PGFEAE respectively

fasting blood glucose (FBG)

fasting insulin (FINS)

insulin sensitivity index (ISI)

serum free fatty acid (FFA) concentration

and triglyceride (TG) concentration were measured. Result: Compared with the normal group

FBG

FINS

FFA

and TG levels were significantly higher and ISI level was lower in model group (P<0.05

P<0.01)

after 4 weeks of modeling

FBG

FINS

and TG levels were 5.94

30.71

0.57 mmol·L-1. 13.05

42.36

1.62 mmol·L-1 respectively in normal group and model group. After 4 weeks of treatment

various dose groups of PGFEAE can effectively reduce FBG

FINS

FFA

and TG levels and increase ISI level in rats with insulin resistance compared with the model group (P<0.05

P<0.01). Conclusion: PGFEAE can effectively improve the symptoms of high blood glucose and blood lipid in rats with insulin resistance.