Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺

冯彬彬; 张建海; 牛小花; 祝慧凤

doi:10.13422/j.cnki.syfjx.2015240018

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Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺

药剂与炮制 | 更新时间：2024-09-23

- Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺
- Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design
- 中国实验方剂学杂志 2015年21卷第24期页码：18-21
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市教委自然科学项目(KJ1402508);巫山庙宇党参产业链工程项目(104290-41003012);重庆三峡医药高等专科学校-西南大学校级横向课题(巫山庙宇党参保健品研发)
- DOI：10.13422/j.cnki.syfjx.2015240018
  中图分类号： R284.2
- 纸质出版日期：2015
- 稿件说明：
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冯彬彬, 张建海, 牛小花, 等. Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺[J]. 中国实验方剂学杂志, 2015,21(24):18-21.

FENG Bin-bin, ZHANG Jian-hai, NIU Xiao-hua, et al. Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 18-21.
冯彬彬, 张建海, 牛小花, 等. Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺[J]. 中国实验方剂学杂志, 2015,21(24):18-21. DOI： 10.13422/j.cnki.syfjx.2015240018.

FENG Bin-bin, ZHANG Jian-hai, NIU Xiao-hua, et al. Optimization of Extraction Technology of Lobetyolin and Total Polysaccharides from Miaoyu Codonopsis Radix by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 18-21. DOI： 10.13422/j.cnki.syfjx.2015240018.

摘要

目的:优选庙宇党参中党参炔苷和党参总多糖的提取工艺条件

为该药材的质量评价提供参考。方法:在单因素试验基础上

采用Box-Behnken试验考察提取温度、超声时间、料液比对庙宇党参中党参炔苷和党参总多糖提取效率的影响。采用RP-HPLC测定党参炔苷的含量

流动相乙腈-水(21:79)

检测波长268 nm;紫外分光光度法测定庙宇党参中总多糖的含量

检测波长490 nm。结果:最佳提取工艺条件为提取时间32 min

料液比1:26

提取温度52℃;党参炔苷提取量和党参总多糖质量分数分别为(78.18±0.21) μg·g-1和(21.02±0.13)%

与预测值的偏差较小。结论:Box-Behnken试验可较好地应用于庙宇党参中党参炔苷和党参总多糖的提取

优选的提取工艺合理、可靠

有效成分提取率高。

Abstract

Objective: To optimize ultrasonic-assisted extraction process of lobetyolin and total polysaccharides from Miaoyu Codonopsis Radix. Method: On the basis of single factor experiments

with yields of lobetyolin and total polysaccharides as indexes

Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions by taking extraction temperature

extracion time and solid-liquid ratio as factors.RP-HPLC and ultraviolet spectrophotometry was used to determine contents of lobetyolin and total polysaccharides

respectively. Result: Optimal extraction technology was as following:extraction temperature of 52℃

extracion time of 32 min and solid-liquid ratio of 1:26.Extracting amount of lobetyolin and mass fraction of total polysaccharides from Miaoyu Codonopsis Radix was(78.18±0.21) μg·g-1 and(21.02±0.13)%

respectively;which was consistent with these predicted values. Conclusion: This optimized extraction process is reasonable and reliable with high yield of effective components.

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