Objective: To investigate the effect of Pingchuanning on B-cell lymphoma/leukemia-2(Bcl-2)

Bcl-2 associated X protein(Bax)

epidermal growth factor(EGF) mRNA

platelet-derived growth factor(PDGF) mRNA expressions

lung function and airway morphology in cold asthma model rats. Method: One hundred and five SD male rats were randomly divided into normal group

model group

Guilong Kechuanning group

dexamethasone group

Pingchuanning high-dose group

medium-dose group and low-dose group with 15 in each group. The ovalbumin sensitization and cold stimulation were adopted to establish the rat asthma model. At the 21st day after the modeling

normal and model groups were given the same amount of distilled water every day

the dexamethasone group(0.4 mg·kg-1·d-1)

Guilong Kechuanning group(10 g·kg-1·d-1) and Pingchuanning high

medium and low dose groups(19.6

9.8

4.9 g·kg-1·d-1) were given corresponding drugs for four weeks. And the changes in lung function of asthma rats were observed

then Bcl-2 and Bax protein expressions of the lung tissues were detected by immunohistochemical assay. The lung histopathological changes were observed by hematoxylin and eosin(HE) staining. Expression of EGF mRNA and PDGF mRNA in the lungs were detected by RT-PCR. Result: Compared with the normal group

the model group showed obvious inflammatory cells invasion

stricture of bronchus

thickening of airway smooth muscle and declined lung function(P<0.01);increases in Bcl-2 protein

EGF mRNA

PDGF mRNA(P<0.01);and decrease in Bax expression(P<0.01). Compared with the model group

all treatment groups showed improvements in the lung function and pathological changes to varying degrees(P<0.01

P<0.05);decreases in Bcl-2 protein

EGF mRNA

PDGF mRNA(P<0.01

P<0.05);and increase in Bax expression(P<0.01

P<0.05). Conclusion: Pingchuanning could reduce the expression of Bcl-2 protein and improve Bax protein in lung of asthmatic rats

inhibit the activation of ERK pathway

alleviate inflammatory reaction

improve the lung function

reduce airway smooth muscle hyperplasia

delay the airway remodeling and treat asthma

by the inhibition of EGF mRNA and PDGF mRNA.