Objective: To explore the antitumor effects and mechanisms of bisdemethoxycurcumin(BDMC) on K562 cells. Method: K562 cells were treated with 1

10

30

50

100μmol·L-1 of BDMC for 48 h or 72 h

then the anti-proliferative effect was assessed by MTT assay.K562 cells were treated with 10

30

50μmol·L-1 of BDMC for 24 h or 48 h

and another blank group was also established. The apoptosis morphology was observed by AO/EB staining using fluorescence microscope

mitochondrial membrane potential(MMP) was detected by Rhodamine 123 staining using flow cytometry instrument and apoptosis rate was detected by Annexin V/PI staining using flow cytometry instrument. The expression of Bcl-2

Bax was evaluated by Western blot method. Result: BDMC could inhibit the proliferation of K562 cells in a dose dependent manner when compared with the blank group. BDMC at the dose of 30

50μmol·L-1 could induce cell apoptosis

decrease the ratio of Bcl-2/Bax and reduce mitochondrial membrane potential. Conclusion: BDMC could inhibit proliferation of K562 cells

and its mechanism may be related with the induction of cell apoptosis via mitochondria pathway.