紫菀酮对脂多糖诱导巨噬细胞释放IL-1, TNF-及NO的影响

王芳; 任刚; 熊永爱; 赵海平; 杨明

doi:10.13422/j.cnki.syfjx.2015240123

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紫菀酮对脂多糖诱导巨噬细胞释放IL-1, TNF-及NO的影响

药理 | 更新时间：2024-09-23

- 紫菀酮对脂多糖诱导巨噬细胞释放IL-1, TNF-及NO的影响
- Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide
- 中国实验方剂学杂志 2015年21卷第24期页码：123-125
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省科技支撑项目(20111BBG70005-4);江西中医药大学青年基金项目(Y027);江西中医药大学重点学科青年教师培养项目(2015jzzdxk012)
- DOI：10.13422/j.cnki.syfjx.2015240123
  中图分类号： R285
- 纸质出版日期：2015
- 稿件说明：
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王芳, 任刚, 熊永爱, 等. 紫菀酮对脂多糖诱导巨噬细胞释放IL-1, TNF-及NO的影响[J]. 中国实验方剂学杂志, 2015,21(24):123-125.

WANG Fang, REN Gang, XIONG You-ai, et al. Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 123-125.
王芳, 任刚, 熊永爱, 等. 紫菀酮对脂多糖诱导巨噬细胞释放IL-1, TNF-及NO的影响[J]. 中国实验方剂学杂志, 2015,21(24):123-125. DOI： 10.13422/j.cnki.syfjx.2015240123.

WANG Fang, REN Gang, XIONG You-ai, et al. Effect of Shionone on IL-1,TNF- and NO Release of Macrophages Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(24): 123-125. DOI： 10.13422/j.cnki.syfjx.2015240123.

摘要

目的:探讨紫菀酮的体外抗炎作用及其机制分析。方法:利用脂多糖诱导RAW264.7巨噬细胞建立细胞炎症反应模型

通过紫菀酮对其进行干预

采用SunBioTM Am-Blue细胞增殖与活性检测试剂检测紫菀酮对RAW264.7巨噬细胞增殖的影响;Griess法检测细胞培养上清液中NO含量;ELISA法检测细胞培养上清液中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量。结果:与空白组比较

50μmol·L-1紫菀酮组RAW264.7巨噬细胞的增殖抑制率明显高于空白组

0.1

30μmol·L-1紫菀酮组RAW264.7巨噬细胞的增殖抑制率无显著性差异;10

30μmol·L-1紫菀酮组NO释放量均显著低于脂多糖组;1

30μmol·L-1紫菀酮组IL-1β和TNF-α释放量均显著低于脂多糖组。结论:紫菀酮呈剂量依赖性抑制脂多糖诱导的RAW264.7巨噬细胞炎性细胞因子IL-1β

TNF-α及NO的释放

具有体外抗炎作用。

Abstract

Objective: To explore anti-inflammatory mechanism in vitro of Shionone. Method: Cellular inflammatory reaction model of RAW264.7 macrophages was induced by lipopolysaccharide

this model was intervened by shionone

effect of shionone on proliferation of RAW264.7 macrophages was detected by SunBioTM Am-Blue cell proliferation and active detection reagent.The content of NO in cell culture supernatants was assessed by Griess reaction

levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were measured by commercially available ELISA kit. Result: Compared with the blank group

proliferation inhibition rate of RAW264.7 macrophages in the 50μmol·L-1 shionone group significantly increased

but this parameter in other shionone groups had no obvious change.Compared with the lipopolysaccharide group

releases of NO in 10

30μmol·L-1 shionone group significantly decreased

levels of TNF-α

IL-β in 1

30μmol·L-1 shionone group significantly decreased. Conclusion: Inhibition of shionone on release of TNF-α

IL-1β and NO shows dose-dependent.Shionone has anti-inflammatory activity in vitro.

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