Objective: To predict the effective components and potential targets of essential oil of Asari Radix et Rhizoma on allergic rhinitis based on serum pharmacochemistry-serum pharmacology and network pharmacology methods. Method: Thirty-six Wistar rats were randomly divided into blank group

essential oil of Asari Radix et Rhizoma 0.5

1 h groups(3 g·kg-1 crude drug)

cetirizine tablets 1 h group(10 mg·kg-1)

prednisone tablets 1 h group(12 mg·kg-1) and Xinqin granules 1 h group(15 g·kg-1)

n=6 in each group. Abdominal aortic blood samples were collected after ig. Sterile separation of serum was done at 3000 r·min-1 and centrifuged for 10 min

then cryopreservedat-20℃. The effect of drug-containing serum sampled at different time points(10% serum volume) on histamine release and hexosaminidase of RBL-2H3(cell density of 2.5×105/mL) was measured by enzyme-linked immunosorbent assay(ELISA)after 1.5 h of antigen stimulation(n=6). Components of drug-containing serum at different time points were detected by gas chromatography-mass spectrometry(GC-MS)

and the transitional components of essential oil of Asari Radix et Rhizoma were determined by comparing the GC-MS fingerprints of essential oil of Asari Radix et Rhizoma

drug-containing serum after 0.5 h

1 h ig as well as blank serum. Then the potential targets of transitional components were predicted to construct and analyze compound-target network. Result: Compared with blank serum group

drug-containing serum sampled at different time points after 0.5

1 h ig of essential oil of Asari Radix et Rhizoma can reduce the total release of histamine and degranulation from RBL-2H3 cells induced by antigen(P<0.05). 12 transitional components in drug-containing serum were detected

ie α-pinene

camphene

2-β-pinene

δ-3-carene

l-limonene

1

8-cineole

eucarvone

borneoll

3

5-dimethoxytoluene

safrole

methyleugenol

and 2

3

5-toluene three oxygen radicals. They may have effects on allergic rhinitis by controlling the targets including prostaglandin G/H synthase 2

muscrinic acetylcholine receptor M3

alpha-1A adrenergic receptor

endothelial nitric-oxide synthase and so on. Conclusion: This method initially reveals the effective components and potential targets of essential oil of Asari Radix et Rhizoma on allergic rhinitis.