女贞苷通过激活AMPK促进脂联素的组装

郭文文; 张志杰; 李珍

doi:10.13422/j.cnki.syfjx.2016010018

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女贞苷通过激活AMPK促进脂联素的组装

药剂与炮制 | 更新时间：2024-01-04

- 女贞苷通过激活AMPK促进脂联素的组装
- Assembly of Adiponectin with Ligustroflavone by Activating AMPK
- 中国实验方剂学杂志 2016年22卷第1期页码：18-21
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家基础科学人才培养基金项目(J1310020)
- DOI：10.13422/j.cnki.syfjx.2016010018
  中图分类号： O657.72;R284
- 纸质出版日期：2016
- 稿件说明：
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郭文文, 张志杰, 李珍. 女贞苷通过激活AMPK促进脂联素的组装[J]. 中国实验方剂学杂志, 2016,22(1):18-21.

GUO Wen-wen, ZHANG Zhi-jie, LI Zhen. Assembly of Adiponectin with Ligustroflavone by Activating AMPK[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 18-21.
郭文文, 张志杰, 李珍. 女贞苷通过激活AMPK促进脂联素的组装[J]. 中国实验方剂学杂志, 2016,22(1):18-21. DOI： 10.13422/j.cnki.syfjx.2016010018.

GUO Wen-wen, ZHANG Zhi-jie, LI Zhen. Assembly of Adiponectin with Ligustroflavone by Activating AMPK[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(1): 18-21. DOI： 10.13422/j.cnki.syfjx.2016010018.

摘要

目的: 检测女贞苷对AMPK[一磷酸腺苷激活的蛋白激酶

adenosine 5'-monophosphate(AMP)-activated protein kinase]的激活作用

探究其激活机制以及对脂联素高聚体组装的影响。方法: 用一定浓度的女贞苷处理人胚肾细胞衍生株293T细胞

通过蛋白质印迹法检测AMPK磷酸化水平

通过HPLC检测细胞中AMP和ATP(三磷酸腺苷

Adenosine 5'-triphosphate)的含量。用女贞苷处理3T3-L1脂肪细胞

检测其对脂联素组装的影响。结果: 女贞苷能够使293T细胞中AMPK磷酸化水平升高。经过女贞苷处理后

细胞中AMP与ATP浓度的比值(AMP/ATP比值)升高。当AMPK表达被沉默后

女贞苷对脂联素高聚体组装的促进作用被解除。结论: 女贞苷能够通过提高细胞中AMP/ATP比值来激活AMPK

进而促进脂联素高聚体的组装。

Abstract

Objective: To investigate activation mechanism of AMPK[adenosine 5'-monophosphate(AMP)-activated protein kinase] by ligustroflavone

and explore effect of ligustroflavone on assembly of adiponectin. Method: 293T cells were treated with ligustroflavone.AMPK phosphorylation and evel of cellular AMP and ATP were examined by western blotting and high performance liquid chromatography(HPLC)

respectively.3T3-L1 adipocyte was treated by ligustroflavone and its effect on assembly of adiponectin was detected. Result: Ligustroflavone can activate AMPK and increase ratio of AMP/ATP in 293T cells.Ligustroflavone also can promote ssembly of adiponectin multimers. Conclusion: Ligustroflavone activates AMPK by increasing ratio of AMP/ATP and promotes adiponectin multimerization by activating AMPK.

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