Objective: To establish the fingerprint of Fufang Jinqiancao granules for evaluating scientifically

controlling the quality and ensuring its production stability. Method: The RP-HPLC was performed on an ODS-BP column with acetonitrile-0.2% phosphoric acid solution as mobile phase

the flow rate was 1 mL·min-1

injection volume was 20μL

the wavelength was set at 350 nm. The temperature of column was maintained at 20℃. Fingerprint Similarity Evaluation Software(edition 2012.130723) of Chinese Pharmacopoeia Commission was used to evaluate the similarity of the 10 batches of Fufang Jinqiancao granules

and to analyze the correlations of 4 ingredients in Fufang Jinqiancao granules. Result: Mangiferin was used as the reference peak

the common mode for the HPLC fingerprints was set up

with the similarities of the 10 batches ranging between 0.961 and 0.997.Altogether 20 common peaks in the chromatograms were found

of which 19 peaks were assigned to herbs. Conclusion: The method is sensitive

accurate and simple

and can be used for the quality control of Fufang Jinqiancao granules.