Objective: To establish a RP-HPLC method for simultaneously determining the contents of chlorogenic acid

caffeic acid forsythin

andrographalide and dehydroandrographalide in Fufang Shuanghua Jujuepian. Method: RP-HPLC was carried out on a hypersil C18(4.6 mm×250 mm

5μm). The mobile phase was A(acetonitrile)-B(0.2% formic acid) in the gradient elution mode(0-8 min

4%-13%A;8-24 min

13%-14%A;24-25 min

14%-26%A;25-65 min

26%-33%A;65-70 min

33%-50%A). The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 240 nm. The column temperature was maintained at 30℃. Result: The calibration curves of chlorogenic acid

caffeic acid

forsythin

andrographolide and dehydroandrographolide were in good linearity within the range of 0.2825-2.8250μg(r=0.9995)

0.0868-0.8675μg(r=0.9997)

0.4800-4.8000μg(r=0.9995)

0.1588-1.5875μg(r=0.9995)

0.1488-1.4875μg(r=0.9994) and the average recoveries were 97.3%(RSD of 1.2%)

97.8%(RSD of 1.1%)

98.9%(RSD of 1.4%)

97.6%(RSD of 1.0%)

99.0%(RSD of 1.5%). Conclusion: The method is simple

reliable and specific

and can be applied for quality control of Fufang Shuanghua Jujuepian.