Objective: To study four different methods for the content determination of anthocyanin in the petroleum ether part

the ethyl acetate part

the n-butyl alcohol part and the water part in Lonicera caerulea var. edulis and its antioxidant activity. Method: L.caerulea var edulis is was extracted by 95% ethanol

dissolved in water after solvent recovery

processed with petroleum ether

ethyl acetate and n-butanol to get four parts of L.caerulea var edulis. Color value method

extinction coefficient method

pH differential method and NaNO2-Al(NO3)3-NaOH colorimetric method were used to determine content of anthocyanin and polyphenols

and the DPPH was used to determine the antioxidant ability of different part in L.caerulea var. edulis

with the detection wavelength at 517 nm. Result: Color value method

extinction coefficient method and pH differential method were used to show the highest anthocyanin content in the part of n-butyl alcohol. The result of NaNO2-Al(NO3)3-NaOH colorimetric method showed that the maximum content of polyphenolsin in the ethyl acetate was 29.45%. The highest antioxidant activity in vitro was found in the ethyl acetate part;the antioxidant activities of n-butyl alcohol part and the water part were similar;the antioxidant activity of the petroleum ether part was the lowest. Conclusion: In L.caerulea var edulis

anthocyanin has a certain antioxidant activity

while polyphenols also showed a high antioxidant activity.