Objective: To optimize the technological parameters in the purification process of active materiomic from Cassia Semen by HPLC fingerprint technique. Method: Using the established HPLC fingerprint of Cassia Semen

the macroporous resin type

loaded concentrations

and elution conditions were optimized using the similarity and recovery rates of five components in fingerprints as indexes. Result: D-101 type resin possessed the highest recovery rates of five components. The optimized purification process was as follows:the loaded concentration was 3 mL

the amount of resin was 10 mL

50 mL of 75% ethanol was used as eluent

respectively. After being purified

the recovery rates of five components were all over 85%

and the similarity of fingerprints was higher than 0.98.These components could get balanced recovery. Conclusion: Based on HPLC fingerprint technique

the optimized purification process of active materiomic from Cassiae Semen with macroporous resin can ensure that the main effective components are consistent with the crude extract of Cassiae Semen.