Objective: To observe the effect of Liuwei Dihuang Wan drug serum on the cells proliferation and expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule excluded-1(ICAM-1) in mesangial cells of rats cultured in high glucose conditions. Method: Different doses of Liuwei Dihuang Wan drug serum were prepared

and rat mesangial cells were cultured under high glucose. grouping:normal group(NG)

high glucose(HG) group

HG+control serum group(10% control serum)

HG+low dose group(2.5% Liuwei Dihuang Wan serum+7.5% control serum)

HG+middle dose group(5% Liuwei Dihuang Wan serum+5% control serum)

HG+high dose group(10% Liuwei Dihuang Wan serum). Following treatment with Liuwei Dihuang Wan drug serum for 24

48 h

mesangial cells activity and proliferation changes were observed with methyl thiazolyl tetrazolium and Brdu incorporation assay. MCP-1 and ICAM-1 secretion in cell culture supernatants were detected by ELISA. MCP-1 and ICAM-1 mRNA expression were measured by Real Time-PCR. Result: Compared with normal group

the cells proliferative activity

MCP-I and ICAM-1 content in supernatant

and the expression of MCP-1 and ICAM-1 mRNA were significantly up-regulated in high glucose group after 24

48 h culture(P<0.01). Compared with the high glucose group

treatment with 5% or 10% Liuwei Dihuang Wan drug serum significantly reversed these above changes(P<0.05)

and the inhibition was in a time and dose-dependent manner. Conclusion: High glucose can induce up-regulation of cells proliferation

MCP-1 and ICAM-1 expression in rat mesangial cells. Liuwei Dihuang Wan may reduce the proliferation activity

decrease MCP-1 and ICAM 1-secretion

and down-regulate MCP-1 and ICAM-1 mRNA expression

thus delaying glomerulosclerosis in diabetic nephropathy.