Objective: To observe the time-toxicity relationship of geniposide on hepatotoxicity and nephrotoxicity in normal rats

and provide scientific basis for the clinical application of gardenia. Method: The 110 Wistar rats were randomly divided into normal group

different time groups(0.5

1

2

4

8

12

24

48

72 h group). The rats in normal group received normal saline by gavage

and the rats in other groups received 1.2 g·kg-1 geniposide by gavage. Orbital venous blood was taken at corresponding time points after gavage

and the serum was taken by centrifugation. aspartate amino transferase(AST)

alkaline phosphatase(ALP)

alkaline phosphatase(ALT)

total bilirubin(TBIL)

urea nitrogen(BUN)

and creatinine(Cr) activities in serum were detected to indicate the toxicity of kidney and liver injury. Result: 12 h after administration of geniposide

AST

ALP

ALT

TBIL

BUN

and Cr levels were significantly higher than those in normal group(P<0.05

P<0.01). 24 h and 48 h after administration of geniposide

AST

ALP

ALT

TBIL

BUN

and Cr levels came to peak values. 72 h after administration of geniposide

AST

ALP

ALT

TBIL

BUN

and Cr levels began to significantly decrease. 240 h after administration

the values basically returned to normal levels. In histopathologic examination

different degree's infiltration of inflammatory cells in portal area

necrosis of liver cells

mild hyperplasia of bile duct in portal area

proliferation of fibrous tissue and other pathological changes were observe. Conclusion: Geniposide at dose of 1.2 g·kg-1 can cause acute liver and kidney injury on rats and show certain time-toxicity relationship.