栀子樟帮法炮制前后的化学成分变化

刘婧; 付小梅; 吴建华; 黄潇; 关志宇; 裴建国; 刘德鸿

doi:10.13422/j.cnki.syfjx.2016020001

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栀子樟帮法炮制前后的化学成分变化

药剂与炮制 | 更新时间：2024-01-04

- 栀子樟帮法炮制前后的化学成分变化
- Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method
- 中国实验方剂学杂志 2016年22卷第2期页码：1-5
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81360620);大学生创新创业教育计划项目(201310412012);江西省卫生厅中医药科研项目(2013A049,2015A040,2015A051);江西省研究生创新专项(YC2014-S291);江西省学位与研究生教育教学改革项目(JXYJG-2012-080);江西中医药大学科研基金项目(2012ZR028,2012ZR057,ZX1012,2013jzzdxk046)
- DOI：10.13422/j.cnki.syfjx.2016020001
  中图分类号： R283.3
- 纸质出版日期：2016
- 稿件说明：
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刘婧, 付小梅, 吴建华, 等. 栀子樟帮法炮制前后的化学成分变化[J]. 中国实验方剂学杂志, 2016,22(2):1-5.

LIU Jing, FU Xiao-mei, WU Jian-hua, et al. Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 1-5.
刘婧, 付小梅, 吴建华, 等. 栀子樟帮法炮制前后的化学成分变化[J]. 中国实验方剂学杂志, 2016,22(2):1-5. DOI： 10.13422/j.cnki.syfjx.2016020001.

LIU Jing, FU Xiao-mei, WU Jian-hua, et al. Comparison of Chemical Composition Change in Gardeniae Fructus Before and After Being Processed with Zhangband Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 1-5. DOI： 10.13422/j.cnki.syfjx.2016020001.

摘要

目的:通过指纹图谱研究栀子经樟帮法炮制前后的化学成分变化

比较其中5种主要活性成分(京尼平苷

京尼平-1-β-D-龙胆双糖苷

西红花苷-Ⅰ

西红花苷-Ⅱ

西红花苷-Ⅲ)的含量差异。方法:采用Hypersil ODS C18色谱柱(4.6 mm×250 mm

5μm)

流动相乙腈-0.2%磷酸水溶液梯度洗脱

流速1.0 mL·min-1

进样量5μL

柱温30℃

DAD检测器采集范围195~700 nm

检测波长238 nm和440 nm。采用"中药色谱指纹图谱相似度评价系统"(2004 A版)进行指纹图谱比较。结果:与生栀子相比

栀子炮制品中5种指标成分的含量均有不同程度的变化

且产生1个新的色谱峰。结论:栀子樟帮法炮制前后化学成分的改变很有可能是导致药效和药性发生改变的物质基础。

Abstract

Objective:To compare chemical composition change in Gardeniae Fructus before and after being processed by fingerprint

and compare content difference of five constituents (geniposide

genipin-1-β-D-gentiobioside

crocin-Ⅰ

crocin-Ⅱ

crocin-Ⅲ) in Gardeniae Fructus. Method:HPLC separation of five constituents was performed on Hypersil ODS C18 column (4.6 mm×250 mm

5μm) with mobile phase of acetonitrile-water (containing 0.2% phosphoric acid) for gradient elution

a flow-rate of 1.0 mL·min-1

injection volume of 5μL

column temperature at 30℃ and detection wavelengthes of 238

440 nm.Chromatograms of 30 batches of samples were compared by software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2004A). Result:Compared with raw products of Gardeniae Fructus

five constituents in processed products were all changed in varying degrees

and one new chromatographic peak were found after processed. Conclusion:Before and after being processed

Chemical composition change in Gardeniae Fructus may be major elements to affect its efficacy and drug properties.

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