Objective:To investigate effects of ultrafiltration process on function of Shentong Zhuyu Tang and investigate feasibility of ultrafiltration technology for purification of this decoction. Method:Painful models were established with hot plate test or acetic acid writhing

inflammatory model were established with daubing dimethylbenzene on auricle.Pain threshold improvement rate of mice

latency of body-writhing

frequency of body-writhing response in 15 minutes and mice auricle swelling inhibition rate were used as indexes to observe effects of ultrafiltration process on analgesic and anti-inflammatory activity of Shentong Zhuyu Tang. Result:Analgesic and anti-inflammatory effects of Shentong Zhuyu Tang was remarkable

the largest percentage of pain threshold extension in mice rose to (106.2±28.1)%

writhing incubation rose to (355.1±42.8) s

number of writhing in 15 minutes rose to 30.8±7.7

auricle swelling inhibitory rate rose to 41.27%

significant difference in all indexes was seen as compared to the control group.No significant difference in all indexes was seen between groups of the non-ultrafiltration processed and ultrafiltration processed by 20

50 nm ultrafiltration membranes. Conclusion:Ultrafiltration technology can be applied to purify Shentong Zhuyu Tang

membrane pore size must be not smaller than 20 nm.