Objective:To analyze the volatile constituents of total lignans from Schisandrae Chinensis Fructus

and study its antitumor and anti-tobacco mosaic virus (TMV) activity. Method:The volatile constituents of total lignans from Schisandrae Chinensis Fructus were analyzed by GC-MS. The anti-TMV and anti-tumor effect were studied by half-leaf necrosis method and MTT method respectively. Result:The 58 compounds were detected from the total lignans from Schisandrae Chinensis Fructus

and 43 of them were identified

and the non-lignans accounted for 67.06% in all identified compounds. The compounds with higher content level included schizandrin (13.974%)

carvopinone (8.064%)

dehydroaromadendrene (6.063%)

valerianol (5.416%)

and gomisin A (4.732%). Their non-lignans components accounted for 67.06%. The anti-TMV and anti-tumor data showed certain inhibitory activity of total lignans from Schisandrae Chinensis Fructus on A549 cells proliferation

and good inhibitory activity on TMV. Conclusion:It is the first time to analyze low polar chemical components of total lignans from Schisandrae Chinensis Fructus by GC-MS

which is simple

fast

accurate and reliable. It provides a more efficient detection method for non-lignans of total lignans from Schisandrae Chinensis Fructus

and also provides a scientific foundation for the study of total lignans on their chemical and biological functions.