Objective:To establish a method of fingerprint analysis on Polygoni Multiflori Radix formula granules by UPLC

identify the main chemical compounds

and provide a scientific method for rapid quality evaluation of Polygoni Multiflori Radix formula granules. Method:Ultra Performance Liquid Chromatography (UPLC) was used on the Agilent Eclipse Plus C18 RRHD (2.1 mm×100 mm

1.8μm)

with acetonitrile-water as the mobile phase for gradient elution. The detective wavelength was set at 254 nm;the flow rate was 0.4 mL·min-1 and the column temperature was maintained at 30℃.Meanwhile

time of flight mass spectroscopy was used to analyze the main chemical compounds in fingerprints. Result:UPLC fingerprints were developed for Polygoni Multiflori Radix formula granules. 12 common peaks were determined

and 5 of them were identified. The similarity was higher than 0.9 between the fingerprints of the different samples of Polygoni Multiflori Radix formula granules and the control fingerprints. Conclusion:The method with good reproducibility is simple

rapid and accurate

which can be used to rapidly and effectively evaluate the quality of Polygoni Multiflori Radix formula granules.