Objective:To establish a measurement method for HPLC fingerprints of Shiduqing capsule

to evaluate the quality stability of Shiduqing capsule

and provide a more scientific and effective method for the quality control of Shiduqing capsule. Method:The fingerprint of Shiduqing capsule was performed on Agilent XDB-C18 column (4.6 mm×250 mm

5μm) with acetonitrile-0.1% phosphoric acid as gradient mobile phase. The flow rate was set at 1.0 mL·min-1. The detection wavelength was set at 220 nm and the column temperature was maintained at 30℃. Traditional Chinese medicine (TCM) chromatographic fingerprint similarity evaluation system (2004AEdition) by State Pharmacopeia Committee of China was used to evaluate the similarity of 10 batches of Shiduqing capsules. Result:The HPLC fingerprint of the Shiduqing capsules was established. Taking baicalin as the reference peak

21 common peaks were found from the compounds. RSD of the relative retention time for each common chromatographic peak was less than 1% and the RSD of relative peak area was less than 4%. The method has good precision

repeatability and stability. With this method to determine the HPLC fingerprints of 10 batches of Shiduqing capsules

the similarity value was more than 0.95 for all of them. Conclusion:The quality of 10 batches of Shiduqing capsules is stable. This method is simple and easy to apply

with good precision

reproducibility and stability

which could manifest the overall quality characteristics of the Shiduqing capsules

and provide a scientific basis for the production and quality control of Shiduqing capsules.