Objective:To establish a method for the determination of quercetin

puerarin

and rutin in flavonoids of Qingping tablet

and provide a reference for the quality control of Qingping tablet. Method:The samples were extracted with ultrasonic extraction method. The Kromasil C18 column(4.6 mm×250 mm

5μm) was used with a mobile phase of methyl alcohol-0.05% TFA for gradient elution. The flow rate was 1.0 mL·min-1

with column temperature of 30℃ and the detection wavelength was 247nm. Result:Puerarin

rutin and quercetin showed good linear relationship within the range of 0.0036-0.1140 g·L-1(r=0.9998)

0.0033-0.1050 g·L-1(r=0.9996) and 0.0032-0.1020 g·L-1(r=0.9997) respectively. The average recovery rate of three components was 99.60% with RSD 0.4%

99.86% with RSD 0.3% and 99.94% with RSD 0.2% respectively. Conclusion:The method is sensitive

simple

repeatable and controllable.It is a better method for determination of flavonoids content in Qingping tablet.