Objective:Preparating resolvability of chemical components of Cyathulae Radix and investigating water decoction and its chemical detached composition of Cyathulae Radix which impact on immune regulation. Method:We adopted the technology of hot water extraction

alcohol sedimentation and macroporous adsorptive resins to establish the split method of chemical components of Cyathulae Radix and four compositions obtained were fatty oils composition

polysaccharide composition

ketosteroid composition and 20% ethanol elution composition

repectively. We adopting the technology of high performance liquid chromatography to study their chemical property and the chemical composition was detached uncross in each composition. Investigating water decoction and its chemical detached composition of Cyathulae Radix which impact the carbon clearance index and the influence of immune organs in mice and in mice serum hemolysin antibody generatin on immune regulation. Result:The results indicated that establishing the detached method based on the chemical components of Cyathulae Radix was practicable

steady and good reproducibility. The characteristic of this method was holo-composition which was detached uncross in each. Cyathulae Radix had the certain effect of immunoenhancement. Conclusion:The effect of immunoenhancement of Cyathulae Radix which was based on polysaccharide and ketosteroid composition.