Objective:To study the effect and mechanism of Kangxianling docotion on renal fibrosis in mice with 5/6 nephrectomy. Method:The 60 C57 mice were randomly divided into sham operation group (n=10) and operation group (n=50). The patients in operation group underwent 5/6 nephrectomy. After two weeks

the patients in operation group were randomly divided into model group

Kangxianling low

medium and high dose groups (0.1

0.2

0.4 mg·kg-1)and rapamycin group(0.16μg·kg-1)

n=10 in each group. In each group were given 0.5 mL normal saline

rapamycin and Kangxianling were respectively given by gavage for the groups after two weeks. Mice were sacrificed after 8 weeks of treatment. 1 day before they were sacrificed

24 h urine was collected to detect 24 h protein

and orbital blood test was taken for serum creatinine

blood urea nitrogen. The remnant kidney was taken to detect α-SMA expression by immunofluorescence method. The type Ⅰ and Ⅲ collagen protein expression in renal tissues was detected by Western blot assay. Result:Compared with the sham group

24-hour urinary protein excretion

serum creatinine

blood urea nitrogen

α-SMA

type Ⅰ and Ⅲ collagen protein expression were significantly increased in the model group (P<0.01).Compared with the model group

24-hour urinary protein excretion

serum creatinine

blood urea nitrogen

α-SMA

type Ⅰ and type Ⅲ collagen expression were decreased in each treatment group(P<0.05). Conclusion:Kangxianling docolion can decrease 24 h urinary protein excretion in mice

improve renal function and delay the occurrence of renal fibrosis

and its mechanism may be related to lowering α-SMA expression and inhibiting type Ⅰ and type Ⅲ collagenl expression in renal tissues.