Objective:To determine the total triterpenes and total sterol content in petroleum ether extract

EtOAc extract

and MeOH extract of the fruiting body of Fomitopsis officinali

and study the anti-tumor activity of three kinds of crude extracts and triterpene monomer compounds isolated from EtOAc extract

and explore the relationship between its anti-tumor activity and the content of total triterpenes and total sterol content. Method:Total terpene and sterol content in three crude extracts was determined by spectrophotometric method. Based on the in vivo anti-tumor experiment in H22 tumor-burdened transplantation mice models

the petroleum ether extract

EtOAc extract

and MeOH extract of the fruiting body as well as triterpene monomer compounds were observed and evaluated for anti-tumor activity. Result:The total triterpenes and total sterol content is the highest in EtOAc extract

followed by petroleum ether extract. Five monomer compounds were isolated and obtained from EtOAc extract:dehydroeburiconic acid

3-keto-dehydrosulphurenic acid

dehydroeburicoic acid

fomlactone A and fomlactone C. In the in vivo experiment

EtOAc fraction has the best anti-tumor activity

and when its concentration reached 1000 mg·kg-1

its anti-tumor rate was 64.65%

closed to the positive drug 5-fluorouracil (anti-tumor rate of 59.98%)

and the immune organ indexes also had some changes. The anti-tumor rate of compound 2(3-keto-dehydrosulfurenic acid) was 75.13%

closed to the positive drug CTX (anti-tumor rate of 81.76%)

highter than positive drug 5-Fu. The other four kinds of monomer compounds all had a certain effect on the tumor. Conclusion:EtOAc extract and five monomer compounds can effectively inhibit tumor growth

and can improve the body's immune system. These five monomer compounds are the effective anti-tumor ingredients of the fruiting body of F. officinali

and the active anti-tumor ingredient may be triterpene compounds in F. officinalis.