Objective:To study the effect of Danggui Beimu Kushen Wan medicated serum on the inhibition of gastric cancer cells SGC-7901and its antineoplastic mechanisms. Method:The 120 Wistar rats were divided into Danggui Beimu Kushen Wan high dose group

middle dose group and low dose group (0.3

0.2

0.1 g·kg-1) and normal group

n=30 in each group

ig 2 times/d

continuous for 7 days to prepare drug serum. The rats whose gastric cancer cells SGC-7901 were treated by blank serum were used as blank group. In the experiment groups

SGC-7901 cells were treated by high dose

middle dose and low dose Danggui Beimu Kushen Wan medicated serum

and then RT-qPCR technique was used to detect the expression level of mRNA of cyclooxygenase-2(COX-2)

Bcl-associated X protein(Bax)

and phosphatase and tensin homolog deleted on chromosome ten(PTEN). Immunocytochemical technique was used to test the protein expression level of COX-2

Bax

and PTEN. Result:Compared with the normal group

high dose group decreased the expression of COX-2 mRNA in SGC-7901 cell by 68%

and increased the expression of Bax and PTEN mRNA by 99.7% and 85.6% respectively (P<0.05). Drug serum can reduce the positive expression rate of COX-2 for SGC-7901 cells

increase its average gray value. increase the positive expression rate of Bax and PTEN

rate and reduce the average gray value (P<0.05). Conclusion:Danggui Beimu Kushen Wan medicated serum could inhibit the expressions of Bax

PTEN and COX-2 at mRNA and protein level in SGC-7901 gastric cancer cells.