D-900型大孔树脂纯化巴戟天低聚糖的工艺优选

杨欣; 宋健平; 关业枝; 王琪; 林励; 林伟栋; 顾善喜

doi:10.13422/j.cnki.syfjx.2016030016

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D-900型大孔树脂纯化巴戟天低聚糖的工艺优选

药剂与炮制 | 更新时间：2024-01-04

- D-900型大孔树脂纯化巴戟天低聚糖的工艺优选
- Optimization of Purification Technology for Oligosaccharides in Morindae Officinalis Radix by D-900 Macroporous Resin
- 中国实验方剂学杂志 2016年22卷第3期页码：16-19
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省科技厅"扬帆计划"引进创新创业团队专项(2014YT02S008);广东省省级科技计划项目(2015A020211021)
- DOI：10.13422/j.cnki.syfjx.2016030016
  中图分类号： R283.6
- 纸质出版日期：2016
- 稿件说明：
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杨欣, 宋健平, 关业枝, 等. D-900型大孔树脂纯化巴戟天低聚糖的工艺优选[J]. 中国实验方剂学杂志, 2016,22(3):16-19.

YANG Xin, SONG Jian-ping, GUAN Ye-zhi, et al. Optimization of Purification Technology for Oligosaccharides in Morindae Officinalis Radix by D-900 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 16-19.
杨欣, 宋健平, 关业枝, 等. D-900型大孔树脂纯化巴戟天低聚糖的工艺优选[J]. 中国实验方剂学杂志, 2016,22(3):16-19. DOI： 10.13422/j.cnki.syfjx.2016030016.

YANG Xin, SONG Jian-ping, GUAN Ye-zhi, et al. Optimization of Purification Technology for Oligosaccharides in Morindae Officinalis Radix by D-900 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 16-19. DOI： 10.13422/j.cnki.syfjx.2016030016.

摘要

目的:优选大孔树脂纯化巴戟天低聚糖的工艺条件

制备高纯度的低聚糖有效部位。方法:以耐斯糖和水溶性低聚糖的吸附-洗脱率为指标

采用单因素试验考察上样液质量浓度、径高比、洗脱剂用量等因素对巴戟天低聚糖大孔树脂纯化工艺的影响。结果:最佳纯化工艺为上样液质量浓度40 g·L-1

树脂柱径高比1:6

上样量与树脂体积比1:25

加水12 BV以流速2 BV·h-1洗脱。水洗脱液中低聚糖平均质量分数、吸附-洗脱率、转移率分别为55.70%

94.45%

91.82%。结论:该纯化工艺简单、稳定、成本低

可应用于中试生产。低聚糖有效部位纯度>50%。

Abstract

Objective: To optimize purification technology of oligosaccharides in Morindae Officinalis Radix by macroporous resin and prepare effective parts of oligosaccharides with high purity. Method: Taking adsorption-desorption rate of nystose and oligosaccharides as indexes

effects of the concentration of sample solution

diameter-height ratio

elution agent and other factors on purification technology of oligosaccharides were investigated by single factor tests. Result: Optimum purification technology was as following:the concentration of sample solution 40 g·L-1

diameter-height ratio of resin column 1:6

ratio of sample volume-resin 1:25

then eluted with 12 BV of water with flow rate of 2 BV·h-1

collected eluent.Average content

adsorption-elution rate and transfer rate of oligosaccharides in water eluent were 55.70%

94.95%

91.82%

respectively. Conclusion: This optimized purification technology is simple and stable with low cost

purity of oligosaccharides is more than 50%.

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