Objective: Endophytes

as a kind of good biological resources

have already produced many metabolites with new structures and various activities. In this paper

during the study of medicinal plant's endophytes

the author isolated and obtained Talaromyceson fungi which could produce diverse metabolites. This paper is to isolate and identify cyclopeptides from this endophytic fungus and study on their acetylcholineterase inhibitory activity. Method: The compounds were isolated by column chromatography methods

including silica gel column chromatography

punching resin column chromatography

and MCI gel chromatography;the structures of compounds were identified using wave spectrum methods

including hydrogen nuclear magnetic resonance (NMR)

carbon nuclear magnetic resonance and electrospray mass spectrometry;acetylcholineterase inhibitory activity of the compounds was detected using improved Ellman method. Result: Four cyclopeptides were isolated and identified as discarine-M(1)

fumitremorgin C(2)

fructigenine B(3)

spirotryprostatin A(4). Compound 1 showed moderate anti-acetylcholineterase activity with IC50 value of 56 μmol·L-1. Conclusion: All of the four cyclopeptides were isolated and obtained from Talaromyces genus for the first time.