Objective: To establish the method for quality control of Qingre Yangwei mixture. Method: The ingredients of Origani Herba and Trollii altaici Flos were identified by thin layer chromatography (TLC). Methylbenzene-ethyl aceate ethyl ethanoate-formic acid (10:7.5:1) was used as developing solvent in the TLC identification of Origani Herba

with caffeic acid as the control product;ethyl aceate ethyl ethanoate-formic acid-water (8:1:0.8) was used as developing solvent in the TLC identification of T. altaici Flos

with rutin and crude T. altaici Flos as the control products.The content of total flavonoids was determined by ultraviolet spectroscopy

and the content of rutin was determined by high performance liquid chromatography (HPLC). Result: The TLC method of Origani Herba and T. altaici Flos from Qingre Yangwei mixture had identification characteristics with clear spots

and showed the same identifying reaction with the control products. In the determination of total flavonoids content

the good linear range was 0.134-0.670 mg for rutin (r=0.999 9)

with an average recovery rate of 98.42% and RSD of 1.2%. In the contents determination

the good linear range was 0.400-4.000 μg for rutin (r=0.999 9)

with an average recovery rate of 98.91% and RSD of 1.2%. Conclusion: This identification method is selective and sensitive. The quantitative method is simple

rapid

accurate and reproducible

and can be used to control the quality of Qingre Yangwei mixture.