Objective: To observe the effects of Sijunzi Tang on glucose absorption and the jejunum mucosa repair in spleen deficiency rats

and investigate the mechanism of Sijunzi Tang for replenishing Qi and invigorating the spleen. Method: The SD rats were divided randomly into 6 groups:normal group

model group

spermidine group(7.5 mg·kg-1) and Sijunzi Tang high dose group

middle dose group and low dose group (22.0

11.0

5.5 g·kg-1). Spleen deficiency models were prepared by reserpine via subcutaneous administration in all rats except normal group. In situ single-pass intestinal perfusion models were used to determine rat intestinal absorption of glucose in different groups by ultraviolet spectroscopy. Light microscope was used to observe the pathological changes of jejunum tissues

and compare the repairing conditions of mucosal injury. Result: The glucose absorption rate constant (Ka) and effective permeability coefficient (Papp) in different groups showed that the glucose absorption was significantly lower in the model group than that in the normal group and the injury of jejunum mucosa was more obvious (P<0.05). Compared with the model group

the glucose absorption was significantly increased in rats of spermidine group and Sijunzi Tang groups

and the injury of jejunum mucosa was significantly repaired (P<0.05). Conclusion: Sijunzi Tang could improve the absorption of glucose via repairing the damaged structure of intestinal mucosa of spleen deficiency rats

which may be one of the possibly important mechanisms for replenishing Qi and invigorating the spleen.