Objective: To investigate the protective effect of medicated serum prepared with Jiangya Baolan tablets on the human umbilical veinendothelial cells (HUVECs) injured by hydrogen peroxide. Method: HUVECs were cultured in vitro and used for experiment. They were divided into five groups as follows:blank group

model group

Jiangya Baolan tablets low dosage group

mid-dosage group and high-dosage group (volume fraction of 5%

10%

15% respectively). The morphological change of the cells was observed under microscope. MTT was used to determine the HUVECs viability. The levels of lactate dehydrogenase (LDH)

and nitric oxide (NO) in supernatant were measured by the microplate method;the levels of endothelin-1(ET-1)

tissue plasminogen activator(tPA) and plasminogen activator inhibitor-1(PAI-1) were determined by enzyme-linked immunosorbent assay (ELISA). Result: Compared with the blank group

the model group significantly decreased HUVECs viability

significantly increased the levels of LDH in supernatant

significantly increased ET-1 and PAI-1

and significantly decreased the levels of NO and tPA (P<0.01). Compared with the model group

Jiangya Baolan tablets mid-dosage and high-dosage groups significantly increased HUVECs viability

significantly decreased the levels of LDH

ET-1

PAI-1

and significantly increased the levels of NO and tPA (P<0.05

P<0.01). Conclusion: Medicated serum prepared with Jiangya Baolan tablets has significant protective effect on HUVECs injured by H2O2.