响应面法优选清喉利咽口含片的提取工艺

王远; 陈良; 刘志雯; 虞慧赟; 姜林

doi:10.13422/j.cnki.syfjx.2016040020

您当前的位置：

首页 >

文章列表页 >

响应面法优选清喉利咽口含片的提取工艺

药剂与炮制 | 更新时间：2024-01-04

- 响应面法优选清喉利咽口含片的提取工艺
- Optimization of Extraction Process of Qinghou Liyan Buccal Tablets by Response Surface Methodology
- 中国实验方剂学杂志 2016年22卷第4期页码：20-23
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016040020
  中图分类号： R283.6
- 纸质出版日期：2016
- 稿件说明：
移动端阅览
王远, 陈良, 刘志雯, 等. 响应面法优选清喉利咽口含片的提取工艺[J]. 中国实验方剂学杂志, 2016,22(4):20-23.

WANG Yuan, CHEN Liang, LIU Zhi-wen, et al. Optimization of Extraction Process of Qinghou Liyan Buccal Tablets by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 20-23.
王远, 陈良, 刘志雯, 等. 响应面法优选清喉利咽口含片的提取工艺[J]. 中国实验方剂学杂志, 2016,22(4):20-23. DOI： 10.13422/j.cnki.syfjx.2016040020.

WANG Yuan, CHEN Liang, LIU Zhi-wen, et al. Optimization of Extraction Process of Qinghou Liyan Buccal Tablets by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 20-23. DOI： 10.13422/j.cnki.syfjx.2016040020.

摘要

目的: 优化院内制剂清喉利咽口含片的提取工艺。方法: 在预试验基础上

以绿原酸、总黄酮(木犀草素-7-O-葡萄糖苷、芦丁、甘草苷)质量浓度的综合评分为因变量

浸泡时间、加水量、提取时间为自变量

采用响应面法优选清喉利咽口含片的提取工艺。采用HPLC测定绿原酸和总黄酮(木犀草素-7-O-葡萄糖苷、芦丁、甘草苷)的含量

流动相乙腈(A)-0.5%磷酸水(B)梯度洗脱(0~12 min

9%A;12~15 min

9%~15%A;15~40 min

15%~45%A;40~50 min

45%~15%A;50~80 min

15%A)

绿原酸和黄酮类成分的检测波长分别为327

256 nm。结果: 最佳提取工艺条件为浸泡时间89 min

液料比15.3 :1

提取时间136 min

提取数2次。绿原酸、木犀草素-7-O-葡萄糖苷、芦丁、甘草苷平均质量浓度分别为1 843.537

184.845

93.939

48.898 mg ·L-1。结论: 建立的数学模型预测性好、稳定可行

可作为清喉利咽口含片的中试生产工艺。

Abstract

Objective: To optimize extraction process of Qinghou Liyan buccal tablets

and provide references for its pilot production. Method: Based on pre-experiments

effects of soaking time

solvent dosage and extracting time on overall desibirability of contents of chlorogenic acid and totale flavonoids (included rutin

liquiritin and luteolin-7-O-glucoside) were investigated and forecasted by response surface metholodgy.HPLC was used to determine contents of chlorogenic acid and three flavonoids with mobile phase of acetonitrile-0.5% phosphoric acid for gradient elution and detection wavelengthes of 327 nm and 256 nm. Result: Optimal extraction process was as following:soaked 89 minutes and extracted twice with 15.3 times the amout of water for per time of 136 minutes.Concentrations of chlorogenic acid

luteolin-7-O-glucoside

rutin and liquiritin were 1 843.537

184.845

93.939

48.898 mg · L-1. Conclusion: This opimized extraction technology is stable and feasible with well prediction

it provides references for pilot production of Qinghou Liyan buccal tablets.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

不同月份三叶青中8种有效成分含量变化

不同干燥方式对桑枝提取物物理指纹图谱及其总黄酮含量的影响

山刺玫不同药用部位中总黄酮的含量测定

HPLC同时测定连翘花及叶中绿原酸等活性成分的含量

HPLC测定金银花及金芪降糖片中6种成分的含量