Objective: To study the chemical constituents from Aconitum tanguticum. Method: The chemical constituents were isolated and purified by using a combination of modern chromatographic techniques

and their structures were determined through spectral analysis(1D

2D-NMR and MS) and literature comparison. Result: Seventeen compounds were isolated and identified as isolumichrome(1)

p-hydroxycinnamic acid(2)

benzoic acid(3)

p-hydroxy phenylethylamine(4)

p-hydroxy phenylethanol(5)

butylparaben(6)

adenosine(7)

3-[p-hydroxyphenyl]-propionic acid amide(8)

hetisine(9)

guanfu base H(10)

salidroside(11)

cyclo-(ile-leu)(12)

phenylalanine(13)

3-(4-aminophenyl)prop-2-enoic acid(14)

N-oxide hordenine(15)

(2R

3R)-2

3

4-trihydroxy-butanoic acid(16)

and N-methyltyramine(17). Conclusion: Compounds 1-4

6-8 and 12-17 were isolated from A. tanguticum for the first time.